NEWS.md
aspect_ratio parameter to all dimensionality reduction plots to control axes ratio of output plot.plot_median and median_size parameters to QC_Plots_* functions.split_collect parameter to FeaturePlot_scCustom to collect all guides when using split.by for a single feature (#94).Clustered_DotPlot to allow modification of sizes of column text labels, legend text labels, and legend title labels (#96).Merge_Sparse_Multimodal_All for merging multi-modal data (1 matrix per modality) (#104).Clustered_DotPlot named row_label_fontface to allow control of fontface used for row labels (#103).Reduction_Loading_Present, in part to fix issue with FeaturePlot_scCustom and internal feature checking.Clustered_DotPlot using new parameters: cluster_feature, cluster_ident (#106).dot_size parameter to statistics plotting functions Plot_Cells_per_Sample and Plot_Median_* family.no_legend to Iterate_Meta_Highlight_Plot to allow for plotting with a plot title instead of plot legend (#108).QC_Plots_Feature to use VlnPlot_scCustom under the hood like rest of QC_Plots_* functions.abort in Meta_Present to return_none to align with Gene_Present and Reduction_Loading_Present.summarise_at, select(.data[[var]]), and rename(.data[[var]]) with current dplyr syntax.Iterate_Cluster_Highlight_Plot and Iterate_Meta_Highlight_Plot to align with recent updates to base Cluster_Highlight_Plot and Meta_Highlight_Plot functions.QC_Plots_Feature to respect parameters when passing to VlnPlot (#91).Read_CellBender_h5_* functions to support CellBender outputs from STARsolo- or Cell Ranger (pre-V3)-processed data (#99).FeaturePlot_scCustom to allow for plotting of dimensionality reduction loadings (#97).Read10X_Multi_Directory and Read10X_h5_Multi_Directory to support files processed with Cell Ranger multi pipeline.Merge_Seurat_List that prevented add.cell.id from adding correct cell name prefixes (#113).label_color_num parameter to PalettePlot allow control of color labeling.Stacked_VlnPlot 90 degrees or 45 (previously possible) (#84).Merge_Seurat_List to avoid ambiguous error messages on failure.Case_Check checks/messages to all feature-based plotting functions.PalettePlot has been changed from palette to pal.PalettePlot to support pal of class “colors”.Cluster_Highlight_Plot and Meta_Highlight_Plot.Fetch_Meta is now S3 generic function that can handle either Seurat or LIGER objects.R/ scripts for better organization.merge parameter to Read10X_GEO, Read10X_h5_GEO, Read_GEO_Delim and Read_CellBender_h5_Multi_File.raster.dpi parameter to DimPlot_LIGER.label parameter to FeaturePlot_scCustom to avoid error collision (#80).vln_linewidth parameter to control violin outline line width (#32).Fetch_Meta for returning data.frame of object meta data.Extract_Sample_Meta to extract sample-level meta data from object.Cell_Highlight_Plot for highlight plots of custom cells not in active ident or meta data.flip parameter to Clustered_DotPlot to enable axes flipping (#69).CellBender_Feature_Diff to return data.frame with count sums and differences between raw and CellBender assays.CellBender_Diff_Plot to plot differences between raw and CellBender assays using data from CellBender_Feature_Diff.mito_name parameter to QC_Plots_Mito to allow for custom specification of meta data column name that contains mitochondrial information.QC_Plots_Combined_Vln() function to return patchwork layout of 3 QC plots.Add_Mito_Ribo_Seurat() and Add_Mito_Ribo_LIGER() (#28).alpha_exp and alpha_na_exp parameters to FeaturePlot_scCustom to allow for control of color scale transparency (#21).*_Highlight_Plot functions can now plot multiple variables simultaneously using either one color for all variables or one color per variable (#34).figure_plot to DimPlot_scCustom(). This removes axes and axes labels and adds axis legend on left bottom corner of plot (#40).plot_legend to Stacked_VlnPlot. This solves issue with returning only one shared legend across all features being plotted (#48).Add_Cell_Complexity_Seurat and Add_Cell_Complexity_LIGER functions to add cell QC complexity/novelty metric (log10(Genes) / log10(UMIs)).QC_Plots_Complexity plot for quick plotting of cell complexity score.Read_CellBender_h5_Mat, Read_CellBender_h5_Multi_Directory, Read_CellBender_h5_Multi_File to enable easy reading of new CellBender output files.raster.dpi parameter from Seurat to all DimPlot FeaturePlot or FeatureScatter based functions.add.noise parameter from Seurat to VlnPlot_scCustom Stacked_VlnPlot functions.group.by as default listed parameter to added to allVlnPlot based QC_Plot_*.ensembl_ids parameter for Add_Mito_Ribo_* functions. If ensembl_ids = TRUE functions will retrieve stored ensembl IDs representing mitochondrial and ribosomal genes for accepted default species.label_feature_yaxis to FeaturePlot_scCustom. Allows for plotting of feature names on secondary y-axis when using split.by (#60).Add_Sample_Meta function for addition of sample-level meta data to cell-level @meta.data slot of Seurat objects.Read_GEO_Delim to check for issues with imported matrices. Check is modified version of SeuratObject::CheckMatrix called CheckMatrix_scCustom(). Will warn if infinite, logical, non-integer (whole), or NA/NaN values are detected in input matrix.QC_Plot_UMIvsGene will now returned filtered correlation value that takes into account meta_gradient_name if provided in addition to nFeature_RNA and nCount_RNA.Variable_Features_ALL_LIGER which allows for detection/selection of variable genes from entire LIGER object instead of iterating by dataset.PackageCheck warning has been added where needed.VlnPlot has been changed to Iterate_VlnPlot_scCustom to reflect that it now uses VlnPlot_scCustom to generate plots.QC_Plot_* functions now use VlnPlot_scCustom internally to unify color scheme and rasterization parameters.*_Highlight_Plot functions no longer display “Unselected” in plot legend and uses DimPlot_scCustom to generate plots (#34).low_cutoff from plotting via QC_Plots_Mito.Clustered_DotPlot that prevented setting identity colors (#29).FeaturePlot_scCustom that returned NULL when setting combine = FALSE (#31).Seq_QC_Plot_* functions which resulted in groups being plotted out of order when specifying plot_by parameter.Seq_QC_Plot_* functions that created color palette error when color palettes were not being used.DimPlot_scCustom that caused mismatch of colors between plots when using split.by if one of the plots was missing 1 or more of the group.by levels (#37).VlnPlot_scCustom that caused raster warning messages to be displayed twice (#42).Iterate_PC_Loading_Plots that caused error when specifying current directory with file_path = NULL or file_path = ""
DotPlot_scCustom that prevented plotting of features in meta.data slot (#44).Stacked_VlnPlot when no supplied features were present.plotFactors_scCustom that was ignoring provided file name.plotFactors_scCustom that caused progress to only display progress up to 50% even when it was fully complete.Clustered_DotPlot that resulted in error related to color palettes if number of clusters was greater than 36 (#49).Add_Mito_Ribo_LIGER that resulted custom column names (e.g. mito_name = "pct.mt") being disregarded and also therefore issue with overwrite parameter. (#51).Store_Misc_Info_Seurat that prevented function from working.Plot_Density_Custom when supplying custom_palette and multiple features. (#51).Clustered_DotPlot so that legend with identities is displayed by factor level of Seurat object idents (#55).Split_FeatureScatter to remove test code that prevented function from working properly (#57).DimPlot_All_Samples, Split_FeatureScatter, and DimPlot_scCustom that ignored factor order when plotting groups.FeaturePlot_scCustom when setting split.by and one or more of features provided was not present in object (#64).VlnPlot_scCustom function.Stacked_VlnPlot
make_unique parameter to Extract_Top_Markers function.Clustered_DotPlot function.Add_Mito_Ribo_Seurat and Add_Mito_Ribo_LIGER.Merge_Sparse_Data_All. (#8)Read_Metrics_10X, Read10X_Multi_Directory, and Read10X_h5_Multi_Directory.StackedVlnPlot with parameters plot_spacing and spacing_unit. (#6)scCustomize_Palette function select palette to use (simplify internal code).